Abstract
Low Power Laser Irradiation therapy raises the levels of proliferation for the variety of cells. Although despite very little is recognized about its effect on the proliferation levels and possible nuclear morphological changes of mice mesenchymal stem cells, which derived from bone marrow and adipose tissue. This study examined the effects of the different fluence of 1, 2, 3 and 4 j/cm² with different dose durations on promoting proliferation, viability and possible nuclear morphological changes of mice mesenchymal stem cells. The growth curves have been used to analyse the proliferation of all the applications. Fluorescent stain (DAPI) (4’-6-diamidino-2-phenylindole) staining used to check the nuclear alterations at 72 hours, while the trypan blue assay used to test the cell viability. Mesenchymal stem cells that derived from bone marrow responded to LPLI in a dose-dependent manner. The higher cell growth determined once the cells irradiated with a fluence of 4 j/cm², especially after 24 hours (P<0. 01). Whereas adipose tissue-derived stem cells responded better to a fluence of 2 j/cm² after 48 hours (P<0. 05). There was no significant change in nuclear alterations and in cell viability detected in the studied groups. LPLI permits a significant increase in the proliferation of stem cells, without causing nuclear alterations, therefore, increasing the number of differentiating cells for tissue engineering, regenerative, and healing processes.
Highlights
The photochemical interaction stems from empirical observation which shall induce chemical effects and reaction inside macromolecules or tissue among the foremost in style created by the evolution if self the energy unharnessed as a result of photosynthesis [1,2]
Depending on the protocol of Soleimani and Nadri [23] The bone marrow stromal cells (BMSCs) and adiposederived stem cells (ADSCS) obtained from three mice with a mean weight of (27 gm), and 60 days age used in this study
The comparison of the four laser fluency show a tendency towards higher cell proliferation within the group treated with laser fluence 4 j/cm2, but the difference was only significant after 72 hours
Summary
The photochemical interaction stems from empirical observation which shall induce chemical effects and reaction inside macromolecules or tissue among the foremost in style created by the evolution if self the energy unharnessed as a result of photosynthesis [1,2]. In the most significant application of photochemical interaction are biostimulation wound healing and anti-inflammatory properties of red or near-infra-red light source as diode laser [3]. Numerous analysis studies have incontestable that Low-Power Laser Irradiation (LPLI) is effective for a few species [5]. The molecular mechanisms of LPLI process are still not completely understood [7,8]. It suggested that the laser energy absorbed by intracellular chromospheres which, converted into metabolic energy and used by a mitochondrial respiratory chain to produce the ATP and reproducible the DNA and synthesis of the RNA and proteins [9,10]
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