Recognition and movement of polystyrene nanoplastics in fish cells

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Although nanoplastics are being increasingly scrutinized, little is known about their kinetic behavior in living organisms, especially in cellular systems. Herein, nonspecific interactions of three polystyrene nanoplastics (pristine-PS, NH2-PS, and COOH-PS, with size range of 90–100 nm and at concentrations of 0–100 μg mL−1) with zebrafish cells were quantified for their cellular uptake and exocytosis. Cell uptake of nanoplastics reached a peak within 2 h and then decreased. The overall nanoplastics uptake was dominated by PS-particle internalization. The estimated uptake rate was comparable among the different types of PS (pristine-PS, NH2-PS, and COOH-PS), but the uptake capacity was related to their functionality. The clathrin-mediated and caveolae-mediated pathways were mainly involved in the uptake of the three nanoplastics. The internalized PS-particles were initially delivered to the cytoplasm but then transported to lysosomes using energy. Meanwhile, these PS particles were released by the cells via energy-free penetration and energy-dependent lysosomal exocytosis. PS-particles were removed by the cells at a relatively slow rate, and the estimated retention half-lives of these PS-particles were 10.1 h, 12.0 h and 15.1 h for pristine-PS, NH2-PS and COOH-PS particles, respectively, in fish cells based on our kinetic measurements. Intracellular trajectory modeling of nanoplastics movement is critical for the environmental and human health risk assessment.