Abstract
Six kinds of chitinous materials have been used as sole carbon/nitrogen (C/N) sources for producing α-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) as the C/N source. The half maximal inhibitory concentration (IC50) and maximum aGI activity of fermented deCSP (38 µg/mL, 98%), deSSP (108 µg/mL, 89%), squid pen powder (SPP) (422 µg/mL, 98%), and shrimp head powder (SHP) (455 µg/mL, 92%) were compared with those of fermented nutrient broth (FNB) (81 µg/mL, 93%) and acarbose (1095 µg/mL, 74%), a commercial antidiabetic drug. The result of the protein/chitin ratio on aGI production showed that the optimal ratio was 0.2/1. Fermented deCSP showed lower IC50 and higher maximum inhibitory activity than those of acarbose against rat intestinal α-glucosidase.
Highlights
Chitin and its derivatives hold great economic value due to their versatile activities and biotechnological applications
TKU042 was investigated with six kinds of chitinous materials: demineralized crab shell powder, demineralized shrimp shell powder, squid pen powder (SPP), shrimp head powder (SHP), fresh shrimp shell powder, and cicada shell powder (CiSP) as the sole sources of C/N with the concentration of 1% (w/v)
To examine whether other bacteria strains, especially the strains of Paenibacillus species, would produce aGI in the demineralized crab shell powder (deCSP)-containing medium, 16 stocked chitinolytic bacteria which were all isolated from Taiwanese soils were tested
Summary
Chitin and its derivatives hold great economic value due to their versatile activities and biotechnological applications. Many microorganisms produce chitinolytic and/or proteolytic enzymes using squid pens as the sole carbon/nitrogen (C/N) source [3,4,13] These findings provide promising results for the production of chitin or chitin oligomers from this chitinous material. Investigations have included the production of enzymes [3,4,5], exopolysaccharides [6,7,8,9], chito-oligomers [3], antioxidants [10], insecticidal materials [11,12], biofertilizers [13], and biosorbents [14,15] These chitinous materials were utilized for another purpose in the present study, the synthesis of antidiabetic drugs via microbial conversion. The half maximal inhibitory concentration (IC50 ) and maximum activity were estimated and compared with those of fermented nutrient broth and acarbose
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
