Abstract
This study reports the effects of aqueous extracts obtained from three fern species of Bulgarian origin: Asplenium ceterach L., Asplenium scolopendrium L., and Asplenium trichomanes L. on the contractility and bioelectrogenesis of rat gastric smooth muscle tissues. In the concentration range 0.015-0.150mg/mL the three extracts contracted smooth muscle tissues in a concentration-dependent manner. The contractions caused by A.ceterach L. and A.scolopendrium L. extracts (0.150mg/mL) were reduced by ketanserin (5×10-7 and 5×10-6mol/L), an antagonist of serotonin 5-HT2 receptor. The contraction evoked by A.trichomanes L. (0.150mg/mL) was significantly reduced by 1×10-6mol/L atropine, an antagonist of muscarinic receptors, and turned into relaxation against the background of 3×10-7mol/L galantamine. After combined pretreatment with galantamine and l-arginine (5×10-4mol/L), this relaxation become more pronounced. The study demonstrates that constituents of A.ceterach L. and A.scolopendrium L. extracts act as agonists of 5-HT2 receptors and cause contraction by activating serotonergic signaling system. A.trichomanes L.-induced reaction is an additive result of two opposite-in-character effects. Thedominant contraction is initiated by inhibition of acetylcholinesterase activity. The relaxation develops with pre-inhibited acetylcholinesterase, it is significantly potentiated by l-arginine, and therefore associated with nitrergic signaling pathway.
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