Receptor mechanisms of prenodal lymphatic constriction by dopamine

Abstract

It has been proposed that alterations in lymphatic smooth muscle activity significantly impact lymphatic function. Numerous endogenous vasoactive agents are known to constrict prenodal lymph vessels. In this study, we assessed the ability of dopamine to alter lymphatic smooth muscle tone in perfused prenodal lymph vessels. Additionally, the receptor mechanisms of dopamine's actions were elucidated. Both intralymphatic (i.l.) and intra-arterial (i.a.) dopamine significantly increased lymphatic perfusion pressure. The increase in lymphatic pressure was completely blocked by i.a. phentolamine, suggesting involvement of α 1- and/or α 2-adrenoreceptors. Intra-arterial infusion of the specific α 1-receptor antagonist prazosin completely abolished the constriction seen during i.l. phenylephrine but only attenuated that produced by dopamine. Intralymphatic infusion of the DA 1-receptor agonist SKF 82526-J and the DA 2-receptor agonist LY 171555 caused significant relaxation of lymph vessels that had been previously constricted by i.a. norepinephrine infusion. These data indicate that the constriction produced by dopamine, in the concentrations employed in this study, is mediated by both α 1- and α 2-adrenoreceptors. These lymph vessels do contain both DA 1- and DA 2-receptors but stimulation of these receptors results in lymphatic smooth muscle relaxation.