Abstract
AbstractMetagenomic approaches are being widely used to detect all culturable and unculturable microbes present in environmental samples in a time-saving and efficient manner. It is applied to perceive the composition of microbes and their activity during the remediation of polluted environments. Metagenomic tools such as next-generation sequencing technologies provide authentic information regarding the major genes and enzymes associated with the degradation and detoxification of contaminants present in soil and water. This chapter highlights the various metagenomic approaches, such as metatranscriptomics, metaproteomics, metabolomics, and fluxomics, used in environmental analysis. This chapter also elaborates on high-throughput next-generation sequencing technologies and the procedures associated with library preparation. The workflow of metagenomics, which is used as targeted and shotgun metagenomics, is discussed. Various examples of microbes in environmental cleanup and the bioinformatic tools utilized in metagenomic data analysis are also elaborated. Finally, the challenges associated with metagenomic approaches are discussed.KeywordsMetagenomicsBioremediationTargeted sequencingShotgun sequencing
Published Version
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