Abstract

The article brings a survey of recent developments in theory and instrumentation for the quantitative evaluation of thin-media chromatograms by photodensitometric measurements. Optical and electrical noise as factors limiting the performance of the method are discussed and attention is called to the possible use of semiconductor photodetectors. Double-beam scanning in its various modifications is considered the best way of reducing the effect of the optical background noise and flying spot scanning is shown to remove errors due to irregular geometry and non-uniform density profiles of the zones. The paper ends with the brief description of a newly developed instrument incorporating these features.

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