Abstract
Formation and properties of chlorophyll-proteolipid particles assembled from Triton-X-100-solubilized spinach thylakoid membrane proteins and monogalactosyldiacylglycerol (MG), or phosphatidylcholine (PC), or phosphatidylethanolamine (PE), were compared. MG particles differed from phospholipid particles in three respects. (1) Chlorophyll proteins were more readily embedded into MG particles than into PE or PC particles. (2) MG particles contained chlorophyll proteins and cytochromes in their in vivo ratios, whereas PE or PC particles were enriched in the light-harvesting chlorophyll a b- protein complex (LHC). (3) As judged by 77 K fluorescence emission spectra and P-700 oxidation kinetics, energy transfer from LHC to reaction center I which was lost in solubilized thylakoids, was restored almost completely in MG particles. In contrast, energy transfer was restored only partially or not at all in proteolipid particles formed with PE or PC, respectively. These unique properties of MG might be linked to its role as major lipid in thylakoid membranes.
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