Abstract
Modification of methods and of a multicellular larval rearing unit for production of Heliothis spp. pupae, particularly the backcross (BC) [BCn ♀ × H. virescens (F.) ♂] from the intercross [ H. virescens ♂ × H. subflexa (Guenee) ♀], are described. Essential components of the unit are a fiber glass tray, a polystyrene insert with 903 cells, and a polypropylene cover with 125-μm openings. More than 10 million BC pupae have been produced during the last 4 years in Stoneville with this unit. Optimum egg density was determined to be two to four eggs per cell. Neither benomyl nor folpet affected growth or development of BC larvae when incorporated into the larval diet at rates from 0.25 to 2.0 g/3.8 liter to control contaminants such as the mold Aspergillus niger (Van Tieghew). The pupal harvesting method collects pupae in a cushioned tray, separates pupae from large particles with a grating, and removes smaller, lighter particles with a highvolume blower.
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