Real-time surface plasmon resonance dispersion imaging with a wide range of incident angles and detection wavelengths

Abstract

We present a wide range of angle- and wavelength-resolved, real-time surface plasmon resonance (SPR) dispersion imaging techniques. Two-dimensional SPR dispersion images were obtained in real time by utilizing a hemicylindrical shaped prism having a high refractive index of 1.72 and a short focal length lens. Our system covers a wide range of incident angles (up to 30) and can detect wavelengths over the whole visible region, allowing us to simultaneously detect the SPR dispersion images produced by air and water substrates (refractive indices of 1 and 1.33, respectively) without scanning or moving the SPR system.