Real-time regulatory effects of IFN-γ and programed death ligand 2 (PDL2) on adherence, proliferation and migration of human placenta-derived mesenchymal stem cells

Abstract

Objective To investigate the real-time regulatory effects of IFN-γ, programed death ligand 2(PDL2) and janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway on the adherence, proliferation and migration of human placenta-derived mesenchymal stem cells(hPMSCs) based on a finding that IFN-γ could enhance the expression of PDL2 in hPMSCs through JAK/STAT signaling pathway. Methods hPMSCs were isolated by using enzyme digestion method and then co-cultured with IFN-γ, anti-PDL2 monoclonal antibody (anti-PDL2 McAb) and JAK inhibitor, respectively. Real-time cell analysis (RTCA) was used to detect the dynamic changes in the adherence, proliferation and migration of hPMSCs following various interventions. Results IFN-γ remarkably suppressed hPMSCs proliferation during the period from 40 hours to 80 hours after intervention and also inhibited the non-targeted migration of hPMSCs. However, hPMSCs adherence was not affected by IFN-γ. Co-culturing hPMSCs with anti-PDL2 McAb significantly enhanced hPMSCs adhesion and inhibited their non-targeted migration, but had no significant effect on hPMSCs proliferation. Furthermore, the proliferation of hPMSCs co-cultured with IFN-γ and anti-PDL2 McAb was significantly inhibited as compared with that of anti-PDL2McAb treatment group. The adhesion, migration and proliferation of hPMSCs were significantly inhibited after co-culturing them with JAK inhibitor. Conclusion IFN-γ can remarkably suppress the proliferation and migration of hPMSCs. PDL2 can enhance the migration and inhibit the adhesion of hPMSCs. JAK/STAT signaling pathway is involved in regulating the adhesion, migration and proliferation of hPMSCs. Key words: IFN-γ; Mesenchymal stem cell; Adherence; Proliferation; Migration; PDL2