Abstract
Homologous recombination in the bacteria cell is regulated at many levels. The central enzyme of homologous recombination RecA and its regulation has been a subject of intense studies by single molecule techniques during the last decade. The RecX protein of Escherichia coli is known to be a negative regulator of RecA. Here we combined optical trapping and microfluidics in order to address the dynamics of RecA-dsDNA filament in the presence of RecX at the single molecule level. Our preliminary results show that RecX blocks elongation of RecA filament and facilitates RecA disassembly from dsDNA in the concentration-dependent manner. The work was carried out with financial support from the Russian Science Foundation (grant No. 14-34-00023) and Russian Foundation for Basic Research (grant No. 13-04-40343-Н).
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