Abstract
Lagovirus europaeus GI.1/GI.2 is an etiological agent causing the highly dangerous rabbit hemorrhagic disease (RHD). Molecular research is the basic tool today that can help solve epidemic problems related to the expansion of pathogens in the world. By using the real-time polymerase chain reaction technique (PCR), we detected three different strains of Lagovirus europaeus/GI.1, which is an RNA virus infecting mainly rabbits. The results showed that the method used was fast, very specific, and effective.
Highlights
Lagovirus (L.) europaeus GI.1 and GI.2 is an etiological agent that causes a severe and highly lethal disease—rabbit hemorrhagic disease (RHD) [1]
Exponential increase in fluorescence was considered to be a positive result of the qualitative real-time polymerase chain reaction technique (PCR) reaction (Figure 1)
The analysis of the results showed that real-time PCR is a highly efficient and effective method for diagnosing L. europaeus GI.1 infections [36]
Summary
Lagovirus (L.) europaeus GI. and GI. is an etiological agent that causes a severe and highly lethal disease—RHD (rabbit hemorrhagic disease) [1]. One of the most commonly used methods is the real-time polymerase chain reaction technique—real-time PCR [4] This method is one of many modifications of the classical PCR reaction and is characterized by the use of a fluorescent dye that allows the analysis of product growth by following the quantitative measurement of the excited fluorescence [4]. In this method, a logarithmic increase in the level of fluorescence is considered a positive result, which determines the increase in the product [4]. An additional advantage is that the method enables both quantitative and qualitative analysis of the material, which makes it possible to significantly shorten and simplify the procedure [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
