Genotyping the human papillomavirus infection in Iranian women referred to Shahid Motahari Hospital, in Urmia, with real-time polymerase chain reaction techniques

Abstract

Objective(s): Human papillomavirus (HPV) is in viridae papillomavirus family. These viruses have been classified based on the DNA sequences. At least 120 types of these viruses have been identified. Different types of HPV genotypes are associated with lower genital tract infection. This study aimed to identify the genotypes of HPV that cause vaginal infections in women with infection. Materials and Methods: This cross-sectional study was performed on eighty patients with vaginal infection. Extracting viral DNA was performed automatically using MagCore Nucleic Acid Extractor (made in Taiwan) and MagCor® Viral Nucleic Acid Extraction kit (Cartridge code 202) made by MogCore Co. Taiwan, and genotyping the samples was carried out using the real-time polymerase chain reaction (PCR) technique in the Light Cycler 96 System (made in Germany) and real-quality relative quantification-HPV infection high-risk (HR)/low-risk (LR) Multiplex Kit made by AB Analitica® Co. Italy. Results: Among the 80 samples, 30 people (37.5%) were positive for infection with HPV; evaluating the positive genotypes, it was found that of these, 16 people (53.4%) were infected with LR genotypes of HPV, seven patients (23.3%) were infected with only HR HPV genotype, and seven people (23.3%) were infected with both LR and HR genotypes of HPV (coinfection). Conclusion: Considering that HPV infection is asymptomatic, and it has a high prevalence in Iran, early diagnosis and prevention of progressing the infection can prevent the malignancies of the uterus. Molecular techniques, particularly real-time PCR, are reliable methods for detecting the HPV infection seven with quite low viral loads.