REAGENTS AND METHODS

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Acta Medica ScandinavicaVolume 157, Issue s322 p. 16-30 REAGENTS AND METHODS First published: January/December 1957 https://doi.org/10.1111/j.0954-6820.1957.tb04134.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Summary I. The reagents are listed. II. The methods are presented and their statistical errors stated. The influence of coagulation and of the presence of dextran is reported and/or discussed. Means are described of avoiding the latter influence in some techniques. In the following list of methods used, the names of the writers whose principles were followed are given in brackets. Prothrombin determination, one-stage (Owren & Aas) and two-stage methods without “accelerin” (Biggs & Douglas) and with “accelerin” (Ware & Seegers). Proaccelerin-accelerin determination, one-stage (Owren) and two-stage method (present writer). Proconvertin-convertin determination, one-stage method (Owren & Aas). Prothrombin consumption test (Brinkhous). Thromboplastin generation test = prothrombinase generation test (Biggs & Douglas). Thrombin generation test (Macfarlane & Biggs). Antithrombin activity determination (Gerendas). The results of the experiments were, inter alia, as follows. The one-stage method of prothrombin determination gave essentially the same results as the two-stage method without “accelerin”. With “accelerin” the values were somewhat higher. This difference occurred in the presence and in the absence of dextran. The change in antithrombin activity during coagulation was independent of the presence of dextran. When accelerin was present, the results with the one-stage method for proaccelerin-accelerin determination were almost entirely dependent on this active factor. Volume157, Issues322January/December 1957Pages 16-30 RelatedInformation