Abstract
Methionyl tRNA binding to wheat embryo ribosomes is catalyzed either by two initiation factors, or by one initiation factor together with a transfer factor. The bound Met-tRNA is reactive with puromycin, however, only when the binding reaction is catalyzed by the two initiation factors. If pactamycin is added to the binding reaction, the bound Met-tRNA is again unreactive with puromycin. These results suggest a two-step sequence for Met-tRNA binding, with puromycin reactivity occurring only as a consequence of the second step. Pactamycin appears to be a specific inhibitor of the reaction converting bound Met-tRNA to puromycin reactivity.
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