Reactivity of monoclonal antibodies directed against lung cancer antigens with human lung, breast and colon cancer cell lines.

Dhia Mukthar,Thomas Schenker,Udo Schumacher

doi:10.1155/1993/619781

Dhia Mukthar, Thomas Schenker + Show 1 more

Open Access

https://doi.org/10.1155/1993/619781

Copy DOI

Abstract

A panel of monoclonal antibodies (n = 72 including controls) directed against lung cancer antigens was screened immunohistochemically against a panel of seven human lung cancer cell lines (including small cell carcinoma, squamous cell carcinoma, adenocarcinoma and mesothelioma), six human breast cancer cell lines and one human colon cancer cell line. The majority of the antibodies (n = 42) reacted also with antigens present on breast and colon cancer cell lines. This cross reactivity especially between lung and breast cancer cell lines is not altogether unexpected since antigens common to breast and lung tissue including their neoplasms such as MUC1 antigen have been described. Our results indicate that epitopes shared by lung and breast cancers are probably more common than previously thought. The relevance for prognosis and therapy of these shared antigens, especially as disease markers in breast cancer, has to be investigated.

Highlights

Some antigens common to lung and breast epithelia can be classified as oncodevelopmental
The details of the results of this study are summarised in Table 2 and can be classified into four groups: 1) those antibodies which did not react with any of the cell lines (n = 14, including the negative controls), 2) those which reacted with breast and colon cancer cell lines only (n = 11), 3) those which reacted with lung cancer cell lines only (n = 17)
The present study has shown that monoclonal antibodies designed for detecting oncodevelopmental antigens expressed in lung cancer can share epitopes of breast and colon cancer cell lines

Summary

Introduction

Some antigens common to lung and breast epithelia can be classified as oncodevelopmental. The MUCI antigen, detected during development in lung, mammary gland and other tissues of epithelial origin (Braga et al, 1992), is conserved during evolution (Welsch et al, 1990, Spicer et al, 1991, Pemberton et al, 1992) and the antigen can be recovered from the human broncho-alveolar-Iavage (Schumacher et at., 1989). Since mucin antigens which are expressed in lung and mammary gland have been described, the monoclonal antibody panel (submitted to the panel of the third international association for the study of lung cancer [IASLC] workshop on lung tumour and differentiation antigens) was tested for cross reactivity with human breast cancer cell lines. Correspondence to: Udo Schumacher, Human Morphology, University of Southampton, Bassett Crescent East, Southampton S09 3TU, U.

Methods

Results

Conclusion