Abstract
SUMMARY. The oxidation of 2, 5‐diniethylfuran (DMF) to cis‐1, 2‐ diacetylethylene (DAE) is a specific test for singlet oxygen (1O2). A method has been developed for the measurement of DAB by direct injection gas chromatography. By the use of this method, the photochemical generation of 1O2 has been demonstrated in samples of two Canadian humic waters.Two other photochemical reactions probably mediated by 1O2generation, the oxidation of histidine and the inactivation of a‐chymotrypsin, have been demonstrated in these waters.The possible ecological and environmental implications of these findings are discussed.
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