Abstract
Peroxynitrite (ONOO−) is one of the sources of oxidation stress involved in many biological signaling pathways. The role of ONOO− being a double-edged sword in biological systems drives the development of effective detection tools. In this work, a boronate-based polymeric fluorescent probe PB-PVA was synthesized and the probe performance was evaluated. The probe exhibits ratiometric sensing of ONOO− in a range of 0–6 µM. There is good linear relationship between the probe fluorescence intensity ratio and ONOO− concentration. The probe also displays moderate selectivity towards ONOO− over other ROS. Moreover, it is water-soluble and possesses good biocompatibility which aids the imaging of ONOO− in living cells. These properties could make the probe a promising tool in in vitro study related to ONOO−.
Highlights
Both reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a double role in physiological pathways
Polyvinyl alcohol (PVA) is of the small molecules water, probe delivery into living cells and the application of together with its high degree of functionality, it is considered as a perfect candidate for the linking incorporated
The probe was subsequently exposed to different ROS to investigate the selectivity model suggests this probe can be applied for ratiometric sensing of ONOO− within the concentration of 0–6 μM
Summary
Both reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a double role in physiological pathways. In the design of the probe, boronates are considered since boronates are recognized as one of the emission bands This method can increase the signal-to-noise ratio and provide a more accurate most effective peroxynitrite-detecting organic molecules [11]. Polyvinyl alcohol (PVA) is of the small molecules water, probe delivery into living cells and the application of together with its high degree of functionality, it is considered as a perfect candidate for the linking incorporated. PB-PVA for the ratiometric of the small molecules in water, facilitating the probe delivery into living cells and the application of. The probe is cell-permeable and displays low cytotoxicity, which facilitates the probe exhibits ratiometric sensing of ONOO− with moderate selectivity and fast response. Design and ONOO− sensing mechanism of the probe PB-PVA
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