Ratio of Yeast Extract Administration on Induction in vitro planlet black orchid (Coelogyne pandurata lindl) From Kalimantan

Abstract

Black Orchid (Coelogyne pandurata) is a flora from Kalimantan that has uniqueness and beauty on the tongue of its flowers. Plants multiply naturally, non-natural propagation has many obstacles, difficult crossing. Plant breeding efforts are required. Tissue culture techniques are expected to be carried out, several endogenous, exogenous factors and the addition of growth regulators (ZPT) are expected to be research efforts for the propagation of this orchid. Efforts to add yeast extract are expected to be a source of growth regulators, because yeast is rich in proteins, free amino acids, peptides, nucleotides, and complex vitamins. This study used the addition of Yeast Extract to the Induction of in Vitro Planlet Black Orchid ( Coelogyne pandurata Lindl) 0.5 g / l, 1 g / l and 1.5 g / l. test analysis using SPSS with Kruskal-Wallis nonparametric test analysis, the results showed that the administration of yeast extract had a significant influence on the number of shoots and the number of roots. A concentration of 1 g/l has an influence on the number of shoots (4.33) and the number of leaves (8.00). A concentration of 1.5 g/l of yeast extract affects the number of roots (3.00).