Pengaruh Paclobutrazol dan Benzyl Adenin terhadap Pertumbuhan dan Multiplikasi Tunas Bawang Merah (Allium cepa L.) Varietas Bima Brebes secara In Vitro

Abstract

In vitro techniques is another way that can provide a number of plants in a relatively quick, free of pathogens, or virus, and available without being affected by season. This research aims to study the effect of paclobutrazol and benzyl adenine on in vitro growth and shoot multiplication of shallot (Allium cepa L. ) varieti Bima Brebes. The experiment was arranged in a Randomized Complete Block Design Group with two factor, the first factor consists of paclobutrazol of four levels (0.0 ppm, 2.5 ppm, 5.0 ppm and 7.5 ppm), and the second factor is the concentration of benzyl adenine consists of three levels (0.0 mg / l, 0.5 mg / l and 1.0 mg / l), in order to obtain 12 combinations of treatments and every treatments consist of 3 repetion. Results from this study indicate that the effect of paclobutrazol and benzyl adenine was no interaction in the number of shoots, leaves, roots, and lenght of rootin of Bima Brebes during 10 MST. The persentage of live explants as a whole is 84.03%. There is no significance effect of paclobutrazol on the number of shoots, number of leaves, number of roots, and leaf lenght shallot of explants. Treatments of benzyl adenin were only have significance effect on the number of shoots and number of leaves shallot of explants, but no significance effect on the number the variable leaf colors, number of roots, and leaf lenght shallot of explants. Keywords : concentration, explants, multiplication, shallot, treatment