Abstract
BackgroundThe question of an age dependence of individual radiosensitivity has only marginally been studied so far. Therefore, we analyzed blood samples of healthy individuals and cancer patients of different ages to determine individual radiosensitivity.MethodsEx vivo irradiated blood samples of 595 individuals were tested. Chromosomes 1, 2 and 4 were stained by 3-color fluorescence in situ hybridization and aberrations were analyzed. Radiosensitivity was determined by the mean breaks per metaphase (B/M).ResultsHealthy individuals (mean age 50.7 years) had an average B/M value of 0.42 ± 0.104 and an increase of 0.0014B/M per year. The patients (mean age 60.4 years) had an average B/M value of 0.44 ± 0.150 and radiosensitivity did not change with age. In previous studies we found that from a value of 0.6B/M on an individual is considered to be distinctly radiosensitive. The portion of radiosensitive individuals (B/M > 0.6) increased in both cohorts with age.ConclusionIndividual radiosensitivity rises continuously with age, yet with strong interindividual variation. No age related increase of radiosensitivity can be demonstrated in patients due to the strong interindividual variation. However among old cancer patients there is a higher probability to have patients with clearly increased radiosensitivity than at younger age.
Highlights
The question of an age dependence of individual radiosensitivity has only marginally been studied so far
Cohort of healthy individuals and cancer patients In total, blood of 595 individuals of different ages was studied for radiosensitivity
The whole cohort consisted of 202 healthy individuals and 393 patients with various solid malignancies like rectal cancer, breast cancer, lung cancer, head and neck tumors, melanoma and prostate cancer (Table 1)
Summary
The question of an age dependence of individual radiosensitivity has only marginally been studied so far. Studies with γH2Ax staining found that γH2Ax foci accumulated with older age reflecting unrepaired DNA damage [3,4,5,6]. It is still unclear whether the remaining DNA double strand breaks result in an. Several studies were able to predict individual radiosensitivity using different techniques [15,16,17,18]. Different time points were used to irradiate lymphocytes. In the G0 assay lymphocytes were irradiated ex vivo in the G0 phase and were stimulated afterwards [14] while in the G2 assay lymphocytes were
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