An Optically Induced Dielectrophoresis (ODEP)-Based Microfluidic System for the Isolation of High-Purity CD45neg/EpCAMneg Cells from the Blood Samples of Cancer Patients-Demonstration and Initial Exploration of the Clinical Significance of These Cells.

Chia-Jung Liao,Yu-Xian Zhu,Tzu-Keng Chiu,Min-Hsien Wu,Chia-Hsun Hsieh,Wen-Pin Chou,Hung-Ming Wang,Feng-Chun Hung

doi:10.3390/mi9110563

Abstract

Circulating tumour cells (CTCs) in blood circulation play an important role in cancer metastasis. CTCs are generally defined as the cells in circulating blood expressing the surface antigen EpCAM (epithelial cell adhesion molecule). Nevertheless, CTCs with a highly metastatic nature might undergo an epithelial-to-mesenchymal transition (EMT), after which their EpCAM expression is downregulated. In current CTC-related studies, however, these clinically important CTCs with high relevance to cancer metastasis could be missed due to the use of the conventional CTC isolation methodologies. To precisely explore the clinical significance of these cells (i.e., CD45neg/EpCAMneg cells), the high-purity isolation of these cells from blood samples is required. To achieve this isolation, the integration of fluorescence microscopic imaging and optically induced dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system was proposed. In this study, an ODEP microfluidic system was developed. The optimal ODEP operating conditions and the performance of live CD45neg/EpCAMneg cell isolation were evaluated. The results demonstrated that the proposed system was capable of isolating live CD45neg/EpCAMneg cells with a purity as high as 100%, which is greater than the purity attainable using the existing techniques for similar tasks. As a demonstration case, the cancer-related gene expression of CD45neg/EpCAMneg cells isolated from the blood samples of healthy donors and cancer patients was successfully compared. The initial results indicate that the CD45neg/EpCAMneg nucleated cell population in the blood samples of cancer patients might contain cancer-related cells, particularly EMT-transformed CTCs, as suggested by the high detection rate of vimentin gene expression. Overall, this study presents an ODEP microfluidic system capable of simply and effectively isolating a specific, rare cell species from a cell mixture.

Highlights

Cancer metastasis is the main cause of cancer-induced death [1]
A comparison of the cancer-related gene expression levels of the CD45neg/EpCAMneg cells isolated from the blood samples of healthy donors and cancer patients was successfully performed
This study proposed the integration of the techniques of fluorescence microscopic observations and optically induced dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system to isolate the specific cells of interest in a high-purity manner

Summary

Introduction

Cancer metastasis is the main cause of cancer-induced death [1]. Circulating tumour cells (CTCs), which have been documented since 1869, are cells that escape from the primary tumour site into the contiguous vasculature and subsequently exist in the blood circulation [2]. In terms of clinical applications, it has been reported that the analysis of CTCs, regarded as a liquid tumour biopsy, can be utilized as a diagnostic or prognostic tool [3] for monitoring cancer metastasis or the therapeutic response [4,5] and for guiding individualized treatment [6]. To achieve these goals, it is necessary to isolate CTCs from a blood sample at high purity to possibly avoid the analytical interferences caused by the surrounding blood cells (mainly leucocytes) [7]

Methods

Results

Conclusion