RATE OF INCREASE IN PLASMA LEVELS OF CYTOPLASMIC AND MITOCHONDRIAL ENZYMES IN EXPERIMENTAL CARBON TETRACHLORIDE HEPATOTOXICITY.

Abstract

Abstract The intraperitoneal administration of carbon tetrachloride to Sprague-Dawley rats, in doses ranging from 0.02 to 0.25 ml. per 100 grams, led to increased plasma levels of glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LD), aldolase (ALD), phosphohexoisomerase (PHI), malic dehydrogenase (MD), isocitric dehydrogenase (ICD), glutathione reductase (GR), and glutamic dehydrogenase (GD). Levels of glucose-6-phosphatase rose inconsistently and to a relatively slight degree. The values of the cytoplasmic enzymes (LD, aldolase, PHI) reached a peak earlier than did those of enzymes that are found in both mitochondria and cytoplasm (GOT, MD, ICD); the most delayed peak was observed with the mitochondrial enzyme (GD). The peak value of GPT, an enzyme with both mitochondrial and cytoplasmic representation, was similar to that of GD, indicating that factors other than cytoplasmic location appear to determine the pattern of response to carbon tetrachloride poisoning.