Comparative Studies on Plasma and Tissue Sorbitol, Glutamic, Lactic and Hydroxybutyric Dehydrogenase and Transaminase Activities in the Dog

Abstract

SUMMARY Plasma and tissue activities in dogs were determined for the following enzymes: sorbitol dehydrogenase (SD), glutamic dehydrogenase (GD), lactic dehydrogenase (LDH), α-hydroxybutyric dehydrogenase (α-HBD), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). After the oral administration of CCl4, the activities of all these enzymes in the plasma were elevated significantly. The measurement of plasma GPT, or SD activity, is recommended in the diagnosis of canine hepatic necrosis because of their tissue specificity, ease of measurement and the marked elevations which accompany liver damage caused by carbon tetrachloride. The clearance of these 6 activities from the plasma was estimated after injection of dog liver homogeuate. GPT, LDH, and α-HBD were cleared from the plasma more rapidly than were the other three activities.