Rat osteogenic sarcoma cells: Comparison of the effects of prostaglandins E 1, E 2, I 2 (prostacyclin), 6-keto F 1α and thromboxane B 2 on cyclic AMP production and adenylate cyclase activity

Abstract

The effects of prostaglandins (PG's) E 1, E 2, I 2 (prostacyclin), 6-keto F 1α and thromboxane (Tx) B 2 were compared in freshly isolated cells from a rat osteogenic sarcoma and in membranes from cultured cells of the same tumour. Cyclic AMP production was measured in cells and adenylate cyclase activity was measured in cell membranes. In both systems PGI 2 was less potent than either of the PGE's, and both TxB 2 and 6-keto PGF 1α were only weak agonists. These effects on bone-derived cells suggest that PGI 2 is unlikely to be a potent bone resorbing agent. Resitance experiments suggested that all the PG's share the same receptor site which appears distinct from the site of action of parathyroid hormone.