Rat liver HMG CoA reductase, a glycoprotein of the endoplasmic reticulum, is in equilibrium between monomeric and dimeric forms

Abstract

Incubation of rat hepatocytes with [ 35S]methionine in pulse and pulsechase experiments followed by immunoprecipitation of the HMG CoA reductase and SDS-PAGE results in two labelled polypeptides of 104 and 180 Kdaltons. These two polypeptides have half lives of 80 and 46 minutes respectively. When hepatocytes are incubated with mevalonolactone, and a pulse of [ 35S]methionine is given, the rate of synthesis of both the 180 and 104 Kd peptides, is strongly diminished. After treatment of the [ 35S] labelled immunoprecipitates with endoglycosidase H, the 180 Kd reductase splits into two labelled peptides of 110 and 97 Kd. We suggest that in addition to the 104 Kd reductase, the endoplasmic reticulum contains the dimer of two reductases linked by a carbohydrate chain. The equilibrium monomer-dimer probably regulates the rate of degradation of reductase.