Abstract
A rapid method is described for the preparation of serum α 1-acid glycoprotein from rats with inflammation induced with turpentine oil injection. The protein obtained by two purification steps, batchwise adsorption with DEAE-cellulose followed by chromatography on CM-cellulose, was proved to be native α 1-acid glycoprotein in a high degree of purity by electrophoretical, immunological, ultracentrifugal and carbohydrate analysis. The monospecific and potent antiserum to this protein was prepared by immunizing rabbits with the desialyzed material emulsified with Freund's incomplete adjuvant. Using purified α 1-acid glycoprotein and its specific antiserum, the concentration of α 1-acid glycoprotein in rat serum was determined by single radial immuno-diffusion. Abnormally high levels of its concentration (5–6 times higher than the control) were observed in inflammatory and tumor bearing rats.
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