Rare HRAS1 alleles outside the VTR region in lymph nodes from patients with malignant lymphoma

Abstract

We found a new type of rare HRAS1 alleles arising de novo in the lymph node of lymphoma patients. Rare alleles of 6.2 kb (a 0.1) and 7.3 kb (a 2.3) are displayed in the presence of common alleles of 6.6 kb (a 1) and 7.6 kb (a 3), respectively. These rare alleles lack nucleotide sequences, present in the common alleles in the 5′ part between the BamHI and XmaIII site. In addition, the rare alleles may have the classic A-G mutation in the intron at position 2719. In contrast to what is observed for rare constitutional alleles, the VTR region of these rare alleles has the same size as the common alleles. At present the significance of this new type of alleles is not clear.