Abstract
Several tests used for the detection of extended-spectrum- b-lactamase (ESBL)-producing enterobacterial isolates have been evaluated in past studies. Detection of ESBLs by the Vitek GNS-506 panel (Bio-Merieux, Marcy l'Etoile, France) is based on the comparison of the reduction in growth by cefo- taxime-clavulanate and ceftazidime-clavulanate with the re- duction caused by the cephalosporins alone. The test has been proven reliable for the detection of ESBLs in Klebsiella pneu- moniae and Escherichia coli and more effective than the dou- ble-disk synergy test (DDST) (3). The Vitek system is widely used in Greek hospitals. In the context of the study of the epidemiology of bacterial resistance in Athens hospitals, we are evaluating randomly selected multidrug-resistant strains in our laboratories. During the course of these studies, we have noticed failure of the Vitek system to detect ESBL in a K. pneumoniae strain. This isolate was resistant to cefoxitin (MIC $ 32 mg/ml) and ceftazidime (MIC $ 32 mg/ml) and susceptible to cefotaxime (MIC # 4 mg/ml). Resistance to cefoxitin indicated the production of plasmidic class C b-lactamase(s). These enzymes are fre- quently encountered among K. pneumoniae and E. coli isolates in Greek hospitals and are closely related to the Citrobacter freundii chromosomal cephalosporinase (1, 2). Testing of the strains by the the disk diffusion method confirmed the resis- tance phenotype. No synergy between ceftazidime or cefo- taxime and amoxicillin-clavulanate (AMC) disks was observed. However, the DDST was positive when AMC was combined with cefepime. The latter antibiotic can be hydrolyzed effi- ciently by various ESBLs but, unlike ceftazidime and cefo- taxime, resists hydrolysis by class C b-lactamases. Subsequent experiments, including isoelectric focusing of crude enzyme preparations from the wild strain and transconjugant clones, showed that the K. pneumoniae isolate simultaneously pro- duced a class C b-lactamase with a highly basic isoelectric point (pI . 9) together with an extended-spectrum enzyme focused at 8.2 (presumably an SHV-5-type b-lactamase). It is likely that the false-negative result obtained with the Vitek ESBL test, and also with the DDST routinely in use in Greek hospitals, was due to interference of the class C b-lac- tamases, which are not inhibited by clavulanate. It should be noted, however, that other K. pneumoniae isolates from the same setting, when examined by Vitek, exhibited similar resis- tance phenotypes but were reported as ESBL positive. There- fore, it may be postulated that the detection system can be deceived when the quantity of AmpC produced, relative to that of ESBL, is such that the presence of the latter enzyme is obscured. The evaluation of this window of error, which is bound to be narrow, is currently under way.
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