Abstract
Quantitative determination of multiple effective components in a given plant usually requires a very large amount of authentic natural products. In this study, we proposed a rapid and non-destructive method for the simultaneous determination of echinacoside, verbascoside, mannitol, sucrose, glucose and fructose in Cistanche tubulosa by near infrared spectroscopy (NIRS). Near infrared diffuse reflectance spectroscopy (DRS) and high performance liquid chromatography (HPLC) were conducted on 116 batches of C. tubulosa samples. The DRS data were processed using standard normal variety (SNV) and multiplicative scatter correction (MSC) methods. Partial least squares regression (PLSR) was utilized to build calibration models for components-of-interest in C. tubulosa. All models were then assessed by calculating the root mean square error of calibration (RMSEC), correlation coefficient of calibration (r). The r values of all six calibration models were determined to be greater than 0.94, suggesting each model is reliable. Therefore, the quantitative NIR models reported in this study can be qualified to accurately quantify the contents of six medicinal components in C. tubulosa.
Highlights
These surveys indicate that phenylethanoid glycosides (PhGs) were the main effective components in Cistanche plants playing key roles for the treatment of kidney deficiency, impotence [8], anti-aging [9] and anti-Alzheimer’s disease [10]
The contents of two PhGs were required in Chinese Pharmacopoeia. Carbohydrates such as mannitol, sucrose, glucose and fructose in Cistanche plants own the laxative function and the carbohydrate clusters of Cistanche plants have been used for the treatment of constipation [11]
The results demonstrated that the developed method could be employed as a reliable method for quantitative analysis of C. tubulosa
Summary
Cistanche tubulosa is an obligate parasite of the roots of perennial plant Tamarix chinensis. In the last three decades, comprehensive and systematic pharmacological studies have been combined with phytochemical investigations to illuminate the material basis of the beneficial effects of the roots of Cistanche plants. These surveys indicate that phenylethanoid glycosides (PhGs) were the main effective components in Cistanche plants playing key roles for the treatment of kidney deficiency, impotence [8], anti-aging [9] and anti-Alzheimer’s disease [10]. Carbohydrates such as mannitol, sucrose, glucose and fructose in Cistanche plants own the laxative function and the carbohydrate clusters of Cistanche plants have been used for the treatment of constipation [11]
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