Rapid Whole Blood Clot Retraction Assay on Quartz Crystal Microbalance

Abstract

This letter demonstrates, for the first time, a novel, rapid automated whole blood clot retraction assay utilizing quartz crystal microbalance resonant frequency monitoring. Tissue factor is selected to initiate coagulation. The test turnaround time is 10–20 min, during which a real-time coagulation trace is recorded in parallel with a reference coagulation trace. The reference sample is the same blood sample that is treated by platelet aggregation inhibitor, and the difference between the two traces indicates the quantitative platelet effect on clotting. This differential approach enables determination of a hematocrit-independent parameter characterizing the intensity of clot retraction. In the case of samples with normal hemostatic characteristics, the intensity of retraction is a linear function of platelet count, whereas the retraction is reduced in the case of platelet hypofunction. Moreover, in combination with a clotting time parameter derived from the same coagulation trace, the method allows primary differentiation between three groups of bleeding disorders of various etiology, including platelet disorders, defects in plasma factors, and hypofibrinogenaemia.