Rapid start of high-concentration denitrification and desulfurization reactors by heterotrophic denitrification sulphur-oxidising bacteria

Abstract

High sulphide concentrations can be toxic to denitrifying and desulphurising microorganisms. In this study, bioaugmentation was used to solve this problem. Pseudomonas sp. gs1 can tolerate 400 mg/L sulphide and converts most of the sulphide into elemental sulphur after 4 h. A solid inoculum of Pseudomonas sp. h1 was prepared. Two reactors, that is, one with and one without inoculum, were simultaneously run for 60 days. Bioreactor II to which bacterial inoculum was added reached a good treatment performance on day 3. The elemental sulphur concentration of the effluent was 342.6 mg/L. It was maintained at 245.3–333.8 mg/L during the subsequent operation. In contrast, reactor I without inoculants achieved the same performance on day 50. High-throughput sequencing shows that Pseudomonas and Azoarcus are the dominant genera. The abundance of the genus Pseudomonas and related denitrifying sulphur-oxidising bacteria in reactor I increases with the operation time. This phenomenon was confirmed by testing the sqr and gltA genes. The quantitative fluorescence PCR test also proves that the addition of bacteria leads to a rapid increase in the sulphur oxidation and carbon metabolism of the activated sludge in the reactor.