Rapid simultaneous spectrophotometric determination of acetaminophen, phenylephrine, and guaifenesin in a cold syrup formulation based on continuous wavelet transform and first derivative transform methods

Abstract

In this study, simultaneous determination of acetaminophen (ACT), phenylephrine (PHE), and guaifenesin (GUA) in synthetic mixtures and syrup formulation was performed using spectrophotometry technique along with continuous wavelet transform (CWT) and derivative transform (DT). Among various wavelet families, Daubechies (Db2), Symlet (Sym4), and Coiflet (Coif2), were selected to analyze ACT, PHE, and GUA, respectively. Mean recovery and root mean square error (RMSE) of CWT method were found 100.19 %, 98.96 %, 95.35 % and 0.00023, 0.0026, 0.0031 for ACT, PHE, and GUA, respectively. For DT method, the first derivative absorption spectra were chosen for determining ACT, PHE, and GUA at 237, 224, and 219 nm, respectively. Mean recovery values were achieved between 97.15 % and 100.35 %. Also, limit of detection (LOD) and limit of quantification (LOQ) were obtained between 0.0138−0.1507, 0.0012−0.0123 μg ml−1 and 0.0695−0.7786, 0.0025−0.0767 μg ml−1 for CWT and DT methods, respectively. These proposed procedures were compared with the high-performance liquid chromatography (HPLC) as a reference technique via analysis of variance (ANOVA). The results did not show a significant difference between the methods. The suggested methods are simple and cost-effective for the quantitative assessment of three-component mixture.