Abstract
A very rapid separation of H1 and core histones by reversed-phase high-performance liquid chromatography using a Bio-Rad Hi-Pore butyl (C 4) silica-based column in a single run is reported. The histones were dissolved in water containing 0.1% trifluoroacetic acid and fractionated within 20 min by means of a linear gradient system consisting of water-acetonitrile containing 0.1% trifluoroacetic acid. For the detection of histones the eluate was monitored at 210 nm. The identity and purity of eluted proteins were confirmed (1) by acid-urea and Triton-acid-urea polyacrylamide gel electrophoresis, and (2) by comparison with the retention times of pure histone markers. Despite the short elution time, a high resolution of the different histone fractions could be obtained. The eluted histones were recovered in the following order: H1 (LHP), H1 (MHP), H2B, H2A (LHP), H4 plus H2A (MHP), H3 (LHP), and H3 (MHP) (where LHP and MHP refer to less hydrophobic and more hydrophobic histone variants). The reported system is preferable to time-consuming electrophoretic systems for the separation of histones.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
