Abstract
Abstract A recently proposed improved rapid screening method for chick edema factor in fats was studied collaboratively by ten laboratories. The new procedure, a modification of the official electron capture method, reduces sample cleanup time by replacing the saponification step of the original procedure with a preliminary sulfuric acid cleanup step. Subsequent cleanup involves alumina column chromatography of the petroleum ether extract from sulfuric acid treatment, and gas chromatographic analysis at 200 °C of a specific polar alumina fraction. Gas chromatographic peaks with retention times vs. aldrin between 10 and 25 indicate the presence of chick edema factor. It is recommended that the modified procedure be adopted as official, first action.
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