Abstract
Abstract An electron capture gas chromatographic screening test has been developed for detecting chick edema factor in fats and fatty acids. The procedure involves extraction of unsaponifiables from 10 g fat, alumina column chromatography of the unsaponifiables, sulfuric acid cleanup of the third alumina fraction (25% ethyl ether in petroleum ether), and electron capture gas chromatography at 200°C. Gas chromatographic peaks with retention times vs. aldrin between 10 and 25 are indicative of thepresence of chick edema factor. The screening test has been evaluated by two collaborative studies, and results indicate that the method is suitable as a screening test for chick edema factor.
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