Abstract
BackgroundWhen novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines. The objective of the study was to collect samples from patients with suspected or confirmed A(H1N1)pdm09 infections that could be made available to the scientific community. Respiratory secretions, sera and peripheral blood mononuclear cells (PBMCs) were collected sequentially (when possible) from patients presenting with suspected or previously confirmed A(H1N1)pdm09 infections. Clinical manifestations and illness outcomes were assessed. Respiratory secretions were tested for the presence of A(H1N1)pdm09 virus by means of isolation in tissue culture and real time RT-PCR. Sera were tested for the presence and level of HAI and neutralizing antibodies against the A(H1N1)pdm09 virus.Findings and conclusionsThirty patients with confirmed A(H1N1)pdm09 infection were enrolled at Baylor College of Medicine (BCM). Clinical manifestations of illness were consistent with typical influenza. Twenty-eight of 30 had virological confirmation of illness; all recovered fully. Most patients had serum antibody responses or high levels of antibody in convalescent samples. Virus-positive samples were sent to J. Craig Venter Institute for sequencing and sequences were deposited in GenBank. Large volumes of sera collected from 2 convalescent adults were used to standardize antibody assays; aliquots of these sera are available from the repository. Aliquots of serum, PBMCs and stool collected from BCM subjects and subjects enrolled at other study sites are available for use by the scientific community, upon request.
Highlights
When novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines
These samples were used to detect and isolate viruses for further characterization and to study the adaptive immune responses following infection. The purpose of this manuscript is to describe the clinical and laboratory features of infection among 30 subjects enrolled at Baylor College of Medicine (BCM) who had confirmed A(H1N1)pdm09 infection, and to inform the research community about the availability of samples collected from subjects enrolled at several study sites, as well as other A(H1N1)pdm09 resources available through the National Institutes of Health (NIH)
Forty-one subjects were enrolled at BCM between May 2009 and July 2009
Summary
When novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines. The purpose of this study was to collect blood and respiratory samples from subjects who were known or suspected to have infection caused by the A(H1N1) pdm virus, and to make available to the scientific community new strains of influenza viruses and other immunologic reagents to facilitate influenza research. These samples were used to detect and isolate viruses for further characterization and to study the adaptive immune responses following infection. The purpose of this manuscript is to describe the clinical and laboratory features of infection among 30 subjects enrolled at Baylor College of Medicine (BCM) who had confirmed A(H1N1)pdm infection, and to inform the research community about the availability of samples collected from subjects enrolled at several study sites, as well as other A(H1N1)pdm resources available through the National Institutes of Health (NIH)
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