Abstract
A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.
Highlights
Rapid Quantitative Turbidimetric Spot Test Analysis of Potassium In Blood SerumMatthieu Tubino*,a, Rafael L. de Souzaa and Nelci F
No interferences of other ions are expected in potassium determination in blood serum, using this reagent
Observing the spectra, where the total “absorbance” must be understood as the sum of the absorbance plus the turbidity, it is easy to conclude that the wavelength where there is the smallest interference of the serum solution, associated with the lowest turbidity is at about 700 to 800 nm
Summary
Matthieu Tubino*,a, Rafael L. de Souzaa and Nelci F. Um método micro-analítico, “spot-test” foi desenvolvido para a determinação de potássio em soro sangüíneo. A medida turbidimétrica é realizada diretamente com esta solução, sem a necessidade de diluição, usando uma micro-cela turbidimétrica desenvolvida neste trabalho. O soro sangüíneo é misturado com o tampão e a solução do regente analítico. Os resultados analíticos obtidos com este método foram comparados com aqueles obtidos usando eletrodo seletivo de potássio. A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode.
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