Abstract
This data article contains data related to the research article entitled “Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice,” published in Biomaterials[1]. Engineered hepatocyte/fibroblast sheets (EHFSs) are used for construction of vascularized subcutaneous liver tissue without a pre-transplant vascularization procedures. Here, we described a rapid production technique of EHFSs by controlling fibroblast density and coating fetal bovine serum (FBS) onto temperature-responsive culture dishes (TRCDs). The human fibroblast monolayer formed on FBS-coated TRCDs within 1 h when seeded at a high density (at least 1.56×105 cells/cm2). The most rapid EHFS production was achieved soon after the adhesion of human primary hepatocytes onto the fibroblast layer.
Highlights
Data accessibilityRapid producing technique Rapid production of engineered human hepatocyte/fibroblast sheet Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan Supplementary data of the article
This data article contains data related to the research article entitled “Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice,” published in Biomaterials [1]
Cosuspensions of hepatocytes and fibroblasts formed Engineered hepatocyte/fibroblast sheets (EHFSs), the EHFSs were often selfdetached from fetal bovine serum (FBS)-coated temperature-responsive culture dishes (TRCDs) without temperature reduction before formation of continuous cell sheet format (Fig. 3B)
Summary
Rapid producing technique Rapid production of engineered human hepatocyte/fibroblast sheet Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan Supplementary data of the article. FBS served as a good TRCD coating for the rapid preparation of fibroblast monolayers. Fibroblast monolayers formed within 1 h by seeding at least 1.56 Â 105 cells/cm. Rapid production of EHFSs was achieved approximately 3 h after the first inoculation of TIG-118 cells
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call