Rapid non-genomic effects of aldosterone on the renal vasculature

Abstract

There is increasing evidence for the importance of rapid non-genomic effects of aldosterone on the human vasculature including renal vessels. Arima and collegues by examining isolated perfused afferent and efferent arterioles from rabbit kidneys found a vasoconstriction in both. In another study the same group showed that endothelium-derived nitric oxide (NO) modulates the vasoconstrictor response to aldosterone in rabbit preglomerular afferent arterioles. Disrupting the endothelium as well as blockade of endothelial NO synthase (eNOS) augmented aldosterone-induced vasoconstriction in this study. Uhrenholt et al. found no effect of aldosterone alone to afferent arterioles but a suppression of depolarisation-induced vasoconstriction. After the blockade of eNOS the aldosterone effect was completely suppressed. In a clinical study in healthy male volunteers injection of aldosterone had no statistically significant effects. Co-infusion of the eNOS inhibitor N(G)-monomethyl- l-arginine ( l-NMMA) changed the effect of aldosterone on renal hemodynamics. Aldosterone in co-infusion with l-NMMA decreased renal plasma flow (RPF) much stronger than l-NMMA alone. Infusion of l-NMMA alone increased GFR whereas aldosterone/ l-NMMA lowered GFR slightly. Aldosterone co-infused with l-NMMA strongly increased renal vascular resistance (RVR). The increase was on top of the smaller increase that was induced by l-NMMA infusion. These data indicate that aldosterone acts via rapid non-genomic effects in vivo in humans at the renal vasculature. Antagonizing the endothelial nitric oxide synthase unmasks these effects. Therefore, rapid non-genomic aldosterone effects increase renal vascular resistance and thereby may mediate arterial hypertension if endothelial dysfunction is present.