Abstract
In this study, a microbiological inhibition method in terms of test tubes was developed for the rapid screening of antibiotics in muscle from porcine, bovine, poultry and fish with a simplified sample pre-treatment method. The detection system in microwell plates consisted of an agar medium mixed with nutrients, sensitizers, indicator bacterium (Geobacillus stearothermophilus ATCC12980) and pH indicator (bromocresol purple). Performance of the present kit was validated for the detection of antibiotics in muscle from different animal species according to ISO13969:2003 guidelines. It indicated that the present kit was sensitive to β-lactams, tetracyclines, macrolides (tylosin) and sulfonamides in muscle from porcine, bovine and poultry at MRL levels established by EU No 37/2010. It was also sensitive to β-lactams, aminoglycosides, tetracyclines, macrolides (erythromycin, tylosin), sulfonamides and lincomycin in muscle from fish at MRL levels established by EU No 37/2010. Moreover, there was a consistence between the validation results and the results on incurred samples for the present kit. Additionally, the present kit was easy to operate, cost-effective and satisfactory in specificity and stability.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
