Abstract
Abstract Spleen is the major secondary lymphoid organ in mammals playing an important role in initiation of adaptive immune response to blood-borne pathogens. Yet, the kinetics of lymphocyte recirculation via spleen in resting and inflammatory conditions remain poorly defined. By perfusing blood and lymphocytes via murine spleen in vitro Ford (Cell Tissue Kinetics 1969) measured accumulation of lymphocytes in circulation over the time of perfusion. I developed a novel mathematical model of lymphocytes recirculation via spleen and fitted that model to Ford's experimental data. The model explains well the majority of experimental data and predicts the average residence time of thoracic duct lymphocytes in the murine spleen of 3 hours. The model predicted that antigenic stimulation of the spleen induces increased migration into the spleen (or increased retention in the spleen) of blood lymphocytes, while cells that recently exited the spleen were nearly not affected. Interestingly, the model was not able to accurately explain the kinetics of lymphocyte migration via the irradiated spleen suggesting that irradiation impacts lymphocyte migration kinetics in a not easily predictable way. Taken together, our analysis provides important insights into kinetics of migration of lymphocytes via spleen.
Published Version
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