Abstract
The incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. Foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. The conventional methods used to detect foodborne pathogen are time consuming and laborious. Hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food analyses. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based methods. This review emphasizes on the principles and application of recent rapid methods for the detection of foodborne bacterial pathogens. Detection methods included are simple polymerase chain reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP) and oligonucleotide DNA microarray which classified as nucleic acid-based methods; optical, electrochemical and mass-based biosensors which classified as biosensor-based methods; enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay which classified as immunological-based methods. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. The developments of rapid detection methods are vital in prevention and treatment of foodborne diseases.
Highlights
Foodborne diseases have become a major public health problem worldwide due to the significantly increased incidence of foodborne diseases over the last 20 years (Oliver et al, 2005)
The results indicated that Probelia® Listeria monocytogenes polymerase chain reaction (PCR) system is as good as the International Organization for Standardization (ISO) method and the detection limit was approximately 20 CFU/mL broth culture for pure culture of L. monocytogenes
Rapid methods are important for the rapid detection of foodborne pathogens in food products to prevent outbreaks of foodborne diseases and the spread of foodborne pathogens
Summary
Foodborne diseases have become a major public health problem worldwide due to the significantly increased incidence of foodborne diseases over the last 20 years (Oliver et al, 2005). PCR have been used in the detection of numerous foodborne pathogens like Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus, Campylobacter jejuni, Salmonella spp. and Shigella spp. Further improvements of mPCR include the development of a novel GeXP-PCR by Zhou et al (2013) for the simultaneous detection of six foodborne bacterial pathogens: Salmonella enterica, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7, Shigella spp. and Campylobacter jejuni. REAL-TIME OR QUANTITATIVE PCR (qPCR) Real-time PCR or quantitative PCR is different from simple PCR whereby it does not require agarose gel electrophoresis for the detection of PCR products This method is able to monitor the PCR products formation continuously in the entire reaction by measuring the fluorescent signal produced by specific duallabeled probes or intercalating dyes. Contaminated fresh meats, 26 h poultry, fish, ready-to-eat salads and bakery products
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
