Abstract
Four isoenzymes of phosphodiesterase were identified in Trimeresurus flavoviridis venom. A rapid and highly reproducible column chromatographic procedure on CM-Sephadex C-25, QAE-Sephadex A-25 and Sephadex G-100 was developed for the purification of these isoenzymes with a yield of 83%. All four isoenzymes are metalloglycoproteins having negligible amounts of phosphomonoesterase activity.
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