Abstract
Abstract A collaborative study was carried out in 36 laboratories to validate a hydrophobic grid membrane filter method for rapid (2-3 days) Salmonella detection by comparing its performance against the AOAC/ BAM reference method. Six products were included in the study: semisweet chocolate, raw ground poultry meat, ground black pepper, cheese powder, egg powder, and nonfat dry milk. With the exception of the naturally contaminated poultry, all products were inoculated in advance with low concentrations of a variety of Salmonella serotypes. The hydrophobic grid membrane filter method detected the following numbers of positive samples (results of the AOAC/BAM reference method are shown in parentheses): chocolate, 67(68); raw ground poultry, 133(131); ground black pepper, 57(56); cheese powder, 81(80); egg powder, 51(45); and nonfat dry milk, 68(72). The 2 methods did not differ significantly at the 95% confidence level in any of the 6 foods. The hydrophobic grid membrane filter method has been adopted official first action for detection of Salmonella in selected foods.
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