Abstract
Abstract The modified fluorometric procedure for nequinate in feed eliminates the need for column separation and is well suited for practical routine feed analysis. Nequinate is extracted from feed with 1% formic acid in CHCl3. The extract is filtered and diluted with CHCl3. Methanesulfonic acid is added to enhance the fluorescence of nequinate and the fluorescence is determined at a 270 nm excitation wavelength and a 372 nm emission wavelength. The average recovery of nequinate from feed is 100.5% with a standard deviation of 2.2%. In the presence of most feed additives, the method is quite specific for nequinate. Other 4-hydroxy quinoline coccidiostats presently used as feed additives produce a low level of fluorescence, but qualitative differences can be detected at low concentrations in the presence of methanesulfonic acid due to the lack of a sharp 270 nm excitation peak.
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