Abstract
A micromethod for the quantitative determination of l-alanine in blood and urine is described. No interference of d-alanine, d-cysteine, l-proline, l-glutamine, l-valine, l-histidine or glycine in concentrations up to 5 mmol/1 was found. Using this method fasting blood levels of l-alanine in children were determined. Defects in gluconeogenesis can be easily established by measuring with this method the in vivo l-alanine metabolism in an oral, or intravenous l-alanine loading test.
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