Abstract
Objective: A model of Near Infrared Diffuse Reflectance Spectroscopy (NIR-DRS) was established for the first time to determine the content of Shengmaxinside I in the honey-fried processing of Rhizoma Cimicifugae. Methods: Shengmaxinside I content was determined by high-performance liquid chromatography (HPLC), and the data of the honey-fried processing of Rhizoma Cimicifugae samples from different batches of different origins by NIR-DRS were collected by TQ Analyst 8.0. Partial Least Squares (PLS) analysis was used to establish a near-infrared quantitative model. Results: The determination coefficient R2 was 0.9878. The Cross-Validation Root Mean Square Error (RMSECV) was 0.0193%, validating the model with a validation set. The Root Mean Square Error of Prediction (RMSEP) was 0.1064%. The ratio of the standard deviation for the validation samples to the standard error of prediction (RPD) was 5.5130. Conclusion: This method is convenient and efficient, and the experimentally established model has good prediction ability, and can be used for the rapid determination of Shengmaxinside I content in the honey-fried processing of Rhizoma Cimicifugae.
Highlights
Rhizoma Cimicifugae is primarily derived from Cimicifuga heracleifolia Kom., Cimicifuga dahurica (Turcz.) Maxim., or Cimicifuga foetida L. [1]
Because the of Shengmaxinside I in the verification sample set is within the calibration set content range, this calibration set and verification set can be used for modeling
Results and Conclusions content of Shengmaxinside I in the verification sample set is within the calibration set content range, this calibration set and verification set can be used for modeling
Summary
Rhizoma Cimicifugae is primarily derived from Cimicifuga heracleifolia Kom., Cimicifuga dahurica (Turcz.) Maxim., or Cimicifuga foetida L. [1]. It is a kind of cool and deconstructive drug commonly used in Chinese traditional medicine. It was first recorded in “Sheng Nong’s herbal classic” and appeared as a “top grade product” drug. It has the effect of clearing rash, heat, and detoxifying and lifting yang [1]. Cimicifugae is suitable for growing in mountains, forests, and roadside grasslands about 2000 m above sea level, and their main producing areas are in the three northeastern provinces of China [2]. Cimicifuga Simplex Wormsk is a perennial herb suitable for growing in warm, humid climates and slightly acidic humus. In the cultivation of Cimicifugae, it is necessary to prevent soil from drought
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