Abstract
The objective of the present research was to establish a rapid analytical method for paeoniflorin and moisture in Xiaoyao Pills (condensed) by near-infrared spectroscopy. The near-infrared spectral data of 97 samples was collected by Nicolet 6700 NIR spectrograph,and the reference value of index component content were obtained by HPLC and oven-drying method. Then the multivariate calibration model of paeoniflorin and moisture were established by patrical least square (PLS) and predicting the content of unknow samples. The results showed that the correlation coefficients (R2) of the quantitative calibration model for paeoniflorin and moisture were 0.99774,0.95352, the root-mean-square error of calibration (RMSEC) were 0.00489,0.132,the root-mean-square error of prediction (RMSEP) were 0.00827,0.177. The results indicated that NIRS can provide a simple and accurate way for the fast determination of index component in large numbers of Xiaoyao Pills (concentrated).
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